Coding

Part:BBa_K2082105:Design

Designed by: Carsten Hain   Group: iGEM16_Bielefeld-CeBiTec   (2016-10-14)


Cytidin deaminase 1 from Pteromyzon marinus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 283


Design Notes

Idea for using K2082105 in our genome wide mutator stems from Badran and Liu, 2015.



Reference

  • Badran, Ahmed H.; Liu, David R. (2015): Development of potent in vivo mutagenesis plasmids with broad mutational spectra. In Nature communications 6, p. 8425. DOI: 10.1038/ncomms9425.


Source

coming soon

References